Cyclization kinetics technique is a high throughput technique for measuring global curvature and flexibility of DNA. It enables to measure features of global geometric and mechanical properties of DNA, including the magnitude and direction of its curvature, helical repeat, and bending and twisting flexibility. Experimental measurements for quantification of DNA bending and flexibility are one of our strengths.
The DNA cyclization assay starts with a linear DNA having two cohesive ends that adhere to form circular DNA following by enzymatic ligation (Figure 1). Since, different DNA sequences have different tendency to bend, the rate of ring closure differ between sequences. We monitor by fluorimeter (Figure 2), the rate of rings closure to calculate the structural properties.
